The immunomagnetic beads were prepared by coupling anti-Escherichia. coli O157:H7 monoclonal antibody with magnetic beads. The colloidal gold test strip was composited with anti-O157:H7 monoclonal antibody marked by colloidal gold as the detection antibody, anti-E.coli O157:H7 polyclonal antibody as the test line and the donkey anti-mouse IgG secondary antibody as the control line. Immunomagnetic separation was combined with colloidal gold lateral flow assay, and this method was applied in the rapid detection of E.coli O157:H7.
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